Supplemental Data for Peng, et al., JBC 279(31): 32941-32949

 

 

PCR primers for amplifying the mouse Id1, Id2, or Id3 coding regions:

 

Mouse Id1 (cloned into the Nhe I/BamH I sites of pHAHA vector)

5'-CTA GCT AGC CAT GAA GGT CGC CAG TGG CAG-3'

5'-CGC GGA TCC TCA GCG ACA CAA GAT GCG ATC-3'

 

Mouse Id2 (cloned into the Nhe I/BamH I sites of pHAHA vector)

5'-CTA GCT AGC CAT GAA AGC CTT CAG TCC GGT G-3'

5'-CGC GGA TCC TTA GCC ACA GAG TAC TTT GCT ATC-3'

 

Mouse Id3 (cloned into the Nhe I/BamH I sites of pHAHA vector)

5'-CTA GCT AGC CAT GAA GGC GCT GAG CCC GGT G-3'

5'-CGC GGA TCC TCA GTG GcA AAA GCT CCT CTT G-3'

 

 

PCR primers for generating siRNA cocktails targeting mouse Id1, Id2, or Id3:

 

Mouse Id1:

5-gcg taa tac gac tca cta tag gATCCTGCAGCATGTAATCGAC-3

5-gcg taa tac gac tca cta tag gAAGTCTCTGGAGGCTGAAAGG-3

 

Mouse Id2:

5-gcg taa tac gac tca cta taGGTGACCAAGATGGAAATCCT-3

5-gcg taa tac gac tca cta tag gCATTCAACGTGTTCTCCTGGT-3

 

Mouse Id3:

5-gcg taa tac gac tca cta tag gAAGGACAAGAGGAGCTTTTGC-3

5-gcg taa tac gac tca cta tag gAACAGCTCTTATGCTGCCTTG-3

 

GFP control:

5'-gcg taa tac gac tca cta tag gTC GAG CTG GAC GGC GAC GTA AAC-3'

5'-gcg taa tac gac tca cta tag gCG GCG GTC ACG AAC TCC AGC AGG-3'

 

 

Primers for quantitative real time-PCR analysis of mouse Id1, Id2, and Id3:

 

Mouse Id1:

5-ACGACATGAACGGCTGCT-3

5-CAGCTGCAGGTCCCTGAT-3

 

Mouse Id2:

5-CGTCTTGCCCAGGTGTCT-3

5-CTCCCCATGGTGGGAATA-3

 

Mouse Id3:

5-GAACTTGGGTGGCCTGAA-3

5-CCACACCCTCTCGACACC-3

 

Mouse GAPDH:

5-GGCTGCCCAGAACATCAT-3

5-CGGACACATTGGGGGTAG-3