PCR primers for amplifying mouse CTGF coding region:

 

5'-CGG GGT ACC ATG GGC ATG CTC GCC TCC GTC GCA GGT CC-3'

5'-CCC AAG CTT ACG CCA TGT CTC CGT ACA TC-3'

 

 

PCR primers for quantitative real-time PCR analysis:

 

Mouse CTGF

5-GTG CCA GAA CGC ACA CTG-3

5-CCC CGG TTA CAC TCC AAA-3

 

Mouse GAPDH

5-GGC TGC CCA GAA CAT CAT-3

5-CGG ACA CAT TGG GGG TAG-3

 

Human CTGF

5-TTG GCC CAG ACC CAA CTA-3

5-GCA GGA GGC GTT GTC ATT-3

 

Human GAPDH

5-CAG CGA CAC CCA CTC CTC-3

5-TGA GGT CCA CCA CCC TGT-3

 

 

PCR primers for generating siRNA cocktail:

 

Human CTGF

5'-gcg taa tac gac tca cta tag gAG GTG TGG CTT TAG GAG CAG T-3'

5'-gcg taa tac gac tca cta tag gCC ATT TCA TGC TTT GAA CGA T-3'

 

Mouse CTGF

5'-gcg taa tac gac tca cta taG GCG TAA AGC CAG GAA GTA AG-3'

5'-gcg taa tac gac tca cta taG GCT CTG ACT CCT GAC AAG TG-3'

 

GFP Control

5'-gcg taa tac gac tca cta tag gTC GAG CTG GAC GGC GAC GTA AAC-3'

5'-gcg taa tac gac tca cta tag gCG GCG GTC ACG AAC TCC AGC AGG-3'