PCR primers for amplifying mouse CTGF coding region:

5'-CGG GGT ACC ATG GGC ATG CTC GCC TCC GTC GCA GGT CC-3'

5'-CCC AAG CTT ACG CCA TGT CTC CGT ACA TC-3'

PCR primers for quantitative real-time PCR analysis:

Mouse CTGF

5’-GTG CCA GAA CGC ACA CTG-3’

5’-CCC CGG TTA CAC TCC AAA-3’

Mouse GAPDH

5’-GGC TGC CCA GAA CAT CAT-3’

5’-CGG ACA CAT TGG GGG TAG-3’

Human CTGF

5’-TTG GCC CAG ACC CAA CTA-3’

5’-GCA GGA GGC GTT GTC ATT-3’

Human GAPDH

5’-CAG CGA CAC CCA CTC CTC-3’

5’-TGA GGT CCA CCA CCC TGT-3’

PCR primers for generating siRNA cocktail:

Human CTGF

5'-gcg taa tac gac tca cta tag gAG GTG TGG CTT TAG GAG CAG T-3'

5'-gcg taa tac gac tca cta tag gCC ATT TCA TGC TTT GAA CGA T-3'

Mouse CTGF

5'-gcg taa tac gac tca cta taG GCG TAA AGC CAG GAA GTA AG-3'

5'-gcg taa tac gac tca cta taG GCT CTG ACT CCT GAC AAG TG-3'

GFP Control

5'-gcg taa tac gac tca cta tag gTC GAG CTG GAC GGC GAC GTA AAC-3'

5'-gcg taa tac gac tca cta tag gCG GCG GTC ACG AAC TCC AGC AGG-3'