Unique enzymes in pMOLUC:
Nco
I C`CATG,G 87
BstE II G`GTNAC,C 95
PflM I CCAN,NNN`NTGG 106
Dra
III CAC,NNN`GTG
106
Bsm
I GAATG,C 7 116
Bsg
I GTGCAG
22/20 116
BsiC I TT`CG,AA 118
BstB I TT`CG,AA 118
EcoR I G`AATT,C 120
BspM I ACCTGC 10/14 138
Sal
I G`TCGA,C 144
Bsu36 I CC`TNA,GG 150
PspA I C`CCGG,G 165
Xma
I C`CCGG,G 165
Sma
I CCC|GGG 167
Bsp120 I G`GGCC,C 168
Apa
I G,GGCC`C
172
BamH I G`GATC,C 181
Mun
I C`AATT,G 186
Bcl
I T`GATC,A 195
Bgl
II A`GATC,T 208
BssH II G`CGCG,C 214
EcoR V GAT|ATC 226
Sph
I G,CATG`C 250
Nhe
I G`CTAG,C 250
Xba
I T`CTAG,A 262
Acc65 I G`GTAC,C 268
Asp718 G`GTAC,C 268
Age
I A`CCGG,T 271
Kpn
I G,GTAC`C
272
Hpa
I GTT|AAC 280
NgoM I G`CCGG,C 288
Nae
I GCC|GGC 290
SnaB I TAC|GTA 296
BspM II T`CCGG,A 303
Stu
I AGG|CCT 319
Avr
II C`CTAG,G 320
Nsi
I A,TGCA`T
330
Not
I GC`GGCC,GC 336
Eag
I C`GGCC,G 336
Xma
III C`GGCC,G 336
Kas
I G`GCGC,C 351
Nar
I GG`CG,CC 352
Ehe
I GGC|GCC 353
Bbe
I G,GCGC`C
355
Mlu
I A`CGCG,T 363
BsiW I C`GTAC,G 366
Spl
I C`GTAC,G 366
Nru
I TCG|CGA 374
Sac
II CC,GC`GG
381
BsrG I T`GTAC,A 387
PaeR7 I C`TCGA,G 396
Xho
I C`TCGA,G 396
Sac
I G,AGCT`C
397
Spe
I A`CTAG,T 406
Msc
I TGG|CCA 413
Eco72 I CAC|GTG 417
Pml
I CAC|GTG 417
Sfi
I GGCCN,NNN`NGGCC 419
Afl
II C`TTAA,G 431
HinD III A`AGCT,T 434
BsmB I CGTCTC 7/11 982
Sap
I GCTCTTC
8/11 1223
Ahd
I GACNN,N`NNGTC 2232
Bsa I GGTCTC
7/11 2293
Bbs
I GAAGAC
8/12 3210
Bbv
II GAAGAC
7/11 3211
Number
of enzymes = 67
The following
enzymes do not cut in pMOLUC:
Asc
I Blp
I BseR
I Bsp1286
I BstX I
EcoN I Esp I Fse I Pme I PpuM I
pMOLUC: sites sorted by name:
Aat
II (2) 145
3154
Acc
I (3) 145
298 1111
Acc65 I (1) 268
Aci
I (41) 335 339
378 380
630 633
644 800
816 871
927 1012
1051 1061
1103 1128
1166 1179
1205 1222
1265 1272
1293 1384
1412 1539
1558 1679
1789 1924
1933 2295
2386 2577
2623 2744
2788 2865
2974 3073
3120
Afl
II (1) 431
Afl
III (3)
363 384 1339
Age
I (1) 271
Aha
II (4) 142
352 2769 3151
Ahd
I (1) 2232
Alu
I (21) 9 31
64 254
348 395
436 477
864 921
932 981
1000 1281
1507 1597
1643 1900
2421 2521
2584
Alw
I (11) 177 188
540 1901
1987 1987 2084
2085
2549
2864 2870
AlwN I (2) 130
1755
Apa I (1)
172
ApaL I (4) 425
1155 1653 2899
Ase I (2)
312 2404
Asp718 (1) 268
Ava
I (2) 165
396
Ava
II (3) 626
2370 2592
Avr
II (1) 320
BamH I (1) 181
Ban
I (3) 268
351 2180
Ban
II (2) 172
397
Bbe
I (1) 355
Bbs
I (1) 3210
Bbv
I (8) 357
941 990 1087
1758 1761
1967 2661
Bbv
II (1) 3211
Bcl
I (1) 195
Bcn
I (9) 167
168 532 680
986 1021
1720 2416
2767
Bfa
I (7) 251
263 321 407
1834 2087
2422
Bgl
I (2) 419
2352
Bgl
II (1) 208
Bpm
I (3) 102
868 2302
Bsa I (1)
2293
BsaA I (3) 296
417 1093
BsaB I (2) 224
538
BsaH I (4) 142
352 2769 3151
BsaJ I (5) 87
165 320 378
1499
BsaW I (5) 271
303 1545 1692
2523
Bsg
I (1) 116
BsiC I (1) 118
BsiE I (7) 222
339 378 1255
1679 2602
2751
BsiHKA I (6) 397
429 1159 1657
2818 2903
BsiW I (1) 366
Bsm
I (1) 116
BsmA I (3) 983
2294 3068
BsmB I (1) 982
BsmF I (2) 153
612
BsoF I (25) 336 339
346 552
633 930
933 979
1076 1129
1245 1263
1266 1384
1539 1682
1747 1750
1956 2284
2473 2623
2650 2745
2974
Bsp120 I (1) 168
BspH I (4) 201
2059 3067 3172
BspM I (1) 138
BspM II (1) 303
Bsr
I (13) 518 648
672 1087
1118 1746
1759 1873
2279 2397
2440 2707
2879
BsrB I (2) 1272
3073
BsrD I (3) 198
2293 2467
BsrG I (1) 387
BssH II (1) 214
BssS I (3) 1512
2896 3203
Bst1107 I (2) 299
1112
BstB I (1) 118
BstE II (1) 95
BstN I (3) 1367
1488 1501
BstU I (14) 216 218
365 374
380 871
940 942
1045 1386
1967 2297
2790 3122
BstY I (9) 181
208 533 1980
1991 2077
2089 2857
2874
Bsu36 I (1) 150
Cac8 I (16) 216 248
252 290
343 350
438 556
598 816
1270 1356
1393 1953
2344 2477
Cfr10 I (3) 271
288 2312
Cla
I (2) 222
358
Csp6 I (7) 68
269 367 388
404 1146
2711
Dde
I (8) 150
609 1149 1614
2023 2189
2729 3155
Dpn
I (18) 183 197
210 221
535 1907
1982 1993
2001 2079
2091 2196
2537 2555
2601 2859
2876 2912
DpnII (18) 181 195
208 219
533 1905
1980 1991
1999 2077
2089 2194
2535 2553
2599 2857
2874 2910
Dra
I (4) 237
2098 2117 2809
Dra
III (1) 106
Drd
I (2) 1034
1447
Dsa
I (2) 87
378
Eae
I (4) 336
411 516 2620
Eag
I (1) 336
Ear
I (2) 1223
3027
Eco47 III (2) 232
595
Eco57 I (2) 1886
2900
Eco72 I (1) 417
EcoO109 I (2) 169
3208
EcoR I (1) 120
EcoR II (3) 1365
1486 1499
EcoR V (1) 226
Ehe
I (1) 353
Fnu4H I (25) 336 339
346 552
633 930
933 979
1076 1129
1245 1263
1266 1384
1539 1682
1747 1750
1956 2284
2473 2623
2650 2745
2974
Fok
I (9) 337
560 622 700
886 1027
2198 2379
2666
Fsp
I (2) 245
2454
Gdi
II (4) 335
337 515 2619
Gsu
I (3) 101
867 2303
Hae
I (5) 319
413 1354 1365
1817
Hae
II (5) 234
355 597 1217
1587
Hae
III (15) 170 319
338 413
422 518
814 1354
1365 1383
1817 2275
2355
2622 3209
Hga
I (5) 876
1035 1449 2027
2759
HgiA I (6) 397
429 1159 1657
2818 2903
HgiE II (2) 1157
1918
Hha
I (20) 216 218
233 246
354 596
942 1045
1075 1216
1249 1519
1586 1686
1860 1969
2362 2455
2792 3124
HinC II (3) 146
280 2773
Hind
II (3) 146
280 2773
HinD III (1) 434
Hinf I (6) 497
895 1239 1314
1710 2227
HinI I (4) 142
352 2769 3151
HinP I (20) 214 216
231 244
352
594 940 1043
1073 1214
1247 1517
1584 1684
1858 1967
2360 2453
2790 3122
Hpa
I (1) 280
Hpa
II (17) 166 272
289 304
531 678
985 1019
1546 1693
1719 1909
2313 2347
2414 2524
2766
Hph
I (8) 106
960 969 2076
2303 2717
2925 2958
Kas
I (1) 351
Kpn
I (1) 272
Mae
I (7) 251
263 321 407
1834 2087
2422
Mae
II (12) 102 142
282 295
369 416
666 1092
2042 2458
2831 3151
Mae
III (14) 95 673
696 780
993 1088
1695 1758
1874 2157
2488 2546
2699 2887
Mbo
I (18) 181 195 208
219
533 1905
1980 1991
1999 2077
2089 2194
2535 2553
2599 2857
2874 2910
Mbo
II (7) 1211
2000 2073 2828
2906 3015
3211
Mlu
I (1) 363
Mme
I (2) 1553
1737
Mnl
I (18) 53 65
145 259
668 726
765 945
975 1237
1447 1520
1771 2171
2252 2398
2604 3199
Msc
I (1) 413
Mse
I (18) 39 236
279 312
432 586
806 838
1120 2045
2097 2102
2116 2169
2404 2443
2808 3180
Msl
I (2) 2484
2643
Msp
I (17) 166 272
289 304
531 678
985 1019
1546 1693
1719 1909
2313 2347
2414 2524
2766
MspA1 I (7) 348
380 932 1051
1681 1926
2867
Mun
I (1) 186
Nae
I (1) 290
Nar
I (1) 352
Nci
I (9) 166 167
531 679
985 1020
1719 2415
2766
Nco I (1)
87
Nde
I (2) 330
1162
NgoM I (1) 288
Nhe
I (1) 250
Nla
III (20) 20 91
139 205
250 388
466 686
750 813
978 1083
1343 2063
2554 2564
2642 2678
3071 3176
Nla
IV (13) 85 170
183 270
353 628
1371 1410
2182 2276
2317 2528
3118
Not
I (1) 336
Nru
I (1) 374
Nsi
I (1) 330
Nsp7524 I (5) 246
384 682 974
1339
NspB II (7) 348
380 932 1051
1681 1926
2867
NspH I (5) 250
388 686 978
1343
PaeR7 I (1) 396
Pal
I (15) 170 319
338 413
422 518
814 1354
1365 1383
1817 2275
2355 2622
3209
PflM I (1) 106
Ple
I (2) 505
1718
Pml
I (1) 417
Psp1406 I (4) 282
666 2458 2831
PspA I (1) 165
Pst I (2)
347 2477
Pvu
I (2) 222
2602
Pvu
II (2) 348
932
Rsa
I (7) 69
270 368 389
405 1147
2712
Sac
I (1) 397
Sac
II (1) 381
Sal
I (1) 144
Sap
I (1) 1223
Sau3A I (18) 181 195
208 219
533 1905
1980 1991
1999 2077
2089 2194
2535 2553
2599 2857
2874
2910
Sau96 I (10) 168 169
420 626
813 2274
2353 2370
2592 3208
Sca
I (2) 405
2712
ScrF I (12) 166 167
531 679
985 1020
1367 1488
1501 1719
2415 2766
Sec
I (5) 87
165 320 378
1499
SfaN I (14) 316 539
547 643
721 783
1006 1139
1177 1215
1435 2487
2680 2927
Sfc I (6)
255 343 490
1604
1795 2473
Sfi
I (1) 419
Sma
I (1) 167
SnaB I (1) 296
Spe
I (1) 406
Sph
I (1) 250
Spl
I (1) 366
Ssp
I (2) 310
3036
Stu
I (1) 319
Sty
I (2) 87
320
Taq
I (8) 33
118 145 222
358 397
1439 2883
Tfi
I (2) 895
1314
Tsp45 I (6) 95
780 993 1088
2488 2699
Tth111 I (2) 101
1086
Tth111 II (4) 801
1928 1937 1967
Vsp
I (2) 312
2404
Xba
I (1) 262
Xca
I (2) 299
1112
Xho
I (1) 396
Xho
II (9) 181
208 533 1980
1991 2077
2089 2857
2874
Xma
I (1) 165
Xma
III (1) 336
Xmn
I (3) 114
899 2831
Site usage in pMOLUC:
Aat
II G,ACGT`C
2 Acc I GT`MK,AC 3
Acc65 I G`GTAC,C
1 Aci
I C`CG,C 41
Afl
II C`TTAA,G
1 Afl
III A`CRYG,T 3
Age
I A`CCGG,T
1 Aha II GR`CG,YC 4
Ahd
I GACNN,N`NNGTC
1 Alu
I AG|CT 21
Alw
I GGATC
8/9 11 AlwN I CAG,NNN`CTG 2
Apa
I G,GGCC`C
1 ApaL
I G`TGCA,C 4
Ase
I AT`TA,AT
2 Asp718 G`GTAC,C 1
Ava
I C`YCGR,G
2 Ava
II G`GWC,C 3
Avr
II C`CTAG,G
1 BamH
I G`GATC,C 1
Ban
I G`GYRC,C
3 Ban II G,RGCY`C 2
Bbe
I G,GCGC`C
1 Bbs
I GAAGAC
8/12 1
Bbv
I GCAGC
13/17 8 Bbv II GAAGAC 7/11 1
Bcl
I T`GATC,A
1 Bcn
I CC,S`GG 9
Bfa
I C`TA,G
7 Bgl
I GCCN,NNN`NGGC 2
Bgl
II A`GATC,T
1 Blp
I GC`TNA,GC -
Bpm
I CTGGAG
22/20 3 Bsa
I GGTCTC
7/11 1
BsaA I YAC|GTR 3
BsaB I
GATNN|NNATC 2
BsaH I GR`CG,YC
4 BsaJ
I C`CNNG,G 5
BsaW I W`CCGG,W
5 BseR
I GAGGAG
16/14 -
Bsg
I GTGCAG
22/20 1 BsiC I TT`CG,AA 1
BsiE I CG,RY`CG
7 BsiHKA
I G,WGCW`C 6
BsiW I C`GTAC,G
1 Bsm
I GAATG,C
7 1
BsmA I GTCTC`/9 3
BsmB I
CGTCTC 7/11 1
BsmF I GGGAC
15/19 2 BsoF I GC`N,GC 25
Bsp120 I G`GGCC,C
1 Bsp1286
I G,DGCH`C -
BspH I T`CATG,A
4 BspM
I ACCTGC
10/14 1
BspM II T`CCGG,A
1 Bsr
I ACT,GG` 13
BsrB I GAG|CGG 2
BsrD I
GCAATG, 8 3
BsrG I T`GTAC,A
1 BssH
II G`CGCG,C 1
BssS I C`TCGT,G
3 Bst1107
I GTA|TAC 2
BstB I TT`CG,AA
1 BstE
II G`GTNAC,C 1
BstN I CC`W,GG
3 BstU
I CG|CG 14
BstX I CCAN,NNNN`NTGG
- BstY
I R`GATC,Y 9
Bsu36 I CC`TNA,GG
1 Cac8
I GCN|NGC 16
Cfr10 I R`CCGG,Y
3 Cla
I AT`CG,AT 2
Csp6 I G`TA,C
7 Dde
I C`TNA,G 8
Dpn
I GA|TC 18 DpnII `GATC, 18
Dra
I TTT|AAA 4
Dra III
CAC,NNN`GTG 1
Drd
I GACNN,NN`NNGTC
2 Dsa
I C`CRYG,G 2
Eae
I Y`GGCC,R
4 Eag
I C`GGCC,G 1
Ear
I CTCTTC
7/10 2 Eco47 III AGC|GCT 2
Eco57 I CTGAAG
21/19 2 Eco72 I CAC|GTG 1
EcoN I CCTNN`N,NNAGG
- EcoO109
I RG`GNC,CY 2
EcoR I G`AATT,C
1 EcoR
II `CCWGG, 3
EcoR V GAT|ATC 1
Ehe I
GGC|GCC
1
Esp
I GC`TNA,GC
- Fnu4H
I GC`N,GC 25
Fok
I GGATG
14/18 9 Fse I GG,CCGG`CC -
Fsp
I TGC|GCA 2
Gdi II
`YGGC,CG 4
Gsu
I CTGGAG
21/19 3 Hae I WGG|CCW 5
Hae
II R,GCGC`Y
5 Hae
III GG|CC 15
Hga
I GACGC
9/14 5 HgiA I G,WGCW`C 6
HgiE II ACCNNNNNNGGT
-1/132 Hha
I G,CG`C
20
HinC II GTY|RAC 3
Hind II GTY|RAC 3
HinD III A`AGCT,T
1 Hinf
I G`ANT,C 6
HinI I GR`CG,YC
4 HinP
I G`CG,C 20
Hpa
I GTT|AAC 1
Hpa II
C`CG,G 17
Hph
I GGTGA
12/11 8 Kas I G`GCGC,C 1
Kpn
I G,GTAC`C
1 Mae I C`TA,G 7
Mae
II A`CG,T
12 Mae III `GTNAC, 14
Mbo
I `GATC, 18
Mbo II
GAAGA 12/11 7
Mlu
I A`CGCG,T
1 Mme I TCCRAC
25/23 2
Mnl
I CCTC
10/10 18 Msc I TGG|CCA 1
Mse
I T`TA,A
18 Msl
I CAYNN|NNRTG 2
Msp
I C`CG,G
17 MspA1
I CMG|CKG 7
Mun
I C`AATT,G
1 Nae
I GCC|GGC 1
Nar
I GG`CG,CC
1 Nci
I CC`S,GG 9
Nco
I C`CATG,G
1 Nde
I CA`TA,TG 2
NgoM I G`CCGG,C
1 Nhe
I G`CTAG,C 1
Nla
III ,CATG`
20 Nla
IV GGN|NCC 13
Not
I GC`GGCC,GC
1 Nru
I TCG|CGA 1
Nsi
I A,TGCA`T
1 Nsp7524
I R`CATG,Y 5
NspB II CMG|CKG 7
NspH I
R,CATG`Y 5
PaeR7 I C`TCGA,G
1 Pal I GG|CC 15
PflM I CCAN,NNN`NTGG
1 Ple
I GAGTC
9/10 2
Pme
I CTTT|AAAC -
Pml I
CAC|GTG 1
PpuM I RG`GWC,CY
- Psp1406
I AA`CG,TT 4
PspA I C`CCGG,G
1 Pst
I C,TGCA`G 2
Pvu
I CG,AT`CG
2 Pvu
II CAG|CTG 2
Rsa
I GT|AC 7 Rsr II CG`GWC,CG -
Sac
I G,AGCT`C
1 Sac II CC,GC`GG 1
Sal
I G`TCGA,C
1 Sap I GCTCTTC
8/11 1
Sau3A I `GATC, 18
Sau96 I
G`GNC,C 10
Sca
I AGT|ACT 2
ScrF I
CC`N,GG 12
Sec
I C`CNNG,G
5 SfaN
I GCATC
9/13 14
Sfc
I C`TRYA,G
6 Sfi
I GGCCN,NNN`NGGCC 1
Sma
I CCC|GGG 1
SnaB I
TAC|GTA 1
Spe
I A`CTAG,T
1 Sph
I G,CATG`C 1
Spl
I C`GTAC,G
1 Srf
I GCCC|GGGC -
Ssp
I AAT|ATT 2
Stu I
AGG|CCT 1
Sty
I C`CWWG,G
2 Taq
I T`CG,A 8
Tfi
I G`AWT,C
2 Tsp45
I `GTSAC, 6
Tth111 I GACN`N,NGTC
2 Tth111
II CAARCA
16/14 4
Vsp
I AT`TA,AT
2 Xba
I T`CTAG,A 1
Xca
I GTA|TAC 2
Xcm I
CCANNNN,N`NNNNTGG-
Xho
I C`TCGA,G
1 Xho
II R`GATC,Y 9
Xma
I C`CCGG,G
1 Xma
III C`GGCC,G 1