THE UNIVERSITY OF CHICAGO MEDICAL CENTER

MOLECULAR ONCOLOGY LABORATORY

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Laboratory Protocols

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 A.  Bacteria, DNA and Molecular Cloning Techniques

 

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A1 Preparation of LB Broth

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A2 Preparation of LB-Agar

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A3 Protocol for Pouring LB-Agar Plates

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A4 Freezing Bacterial Cells for Long Term Storage

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A5 Preparation of electrocompetent DH10B bacterial cells

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A6 Preparation of electrocompetent XL-1 Blue bacterial cells

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A7 Preparation of electrocompetent BJAdEasy1 bacterial cells

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A8 Preparation of electrocompetent BJ5183 bacterial cells

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A9 Checking Competency of Electrocompetent Bacterial Cells

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A10 Transformation of electrocompetent DH10B or XL-1 bacterial cells

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A11 Transformation of BJAdEasy bacterial cells

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A12 Transformation of BJ5183 bacterial cells

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A13 Harvesting Bacteria from Plates

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A14 Alkaline Lysis Miniprep of Plasmid DNA

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A15 Large Scale Plasmid Purification (CsCl Banding)

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A16 Purification of DNA using Wizard plus miniprep kit

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A17 Bacterial Colony Lifts for Cloning Screening

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A18 MOLab Standard DNA Cloning/Ligation Protocol

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A19 T4 DNA Pol blunt-ending or fill-in reactions

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A20 Protocol for PC8 Extraction of DNA Samples

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A21 Protocol for Ethanol Precipitation of DNA Samples

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A22 Isopropanol Precipitation Removing Small Oligomers

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A23 Preparation of Sephadex G-25 and Sephacryl S-300

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A24 Preparing and Using Homemade Spin Columns for DNA Purification from Gels

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A25 Protocol for large scale restriction digestion reactions

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A26 SYBR I Green Staining for low amount of DNA

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A27 Multimerization of Oligonucleotides

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A28 DNA and Oligo Labeling with T4 kinase

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A29 DNA Labeling with Random Priming (Oligo labelling)

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A30 Electrophoretic Mobility Shift Assay (EMSA)

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A31 DNA Cycle Sequencing Protocol

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A32 Preparing and Running Genomyx Sequencing Gel

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A33 Preparation of SSSSS DNA

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A34 Preparation of Miniprep Plasmid for DNA Sequencing

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A35 Protocol of Determining DNA, RNA, and Oligo Concentration

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A36 DNA laddering apoptosis assay

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A37 Extract DNA From Agarose Gel Using Montage DNA Gel Extraction Device

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A38 How to Download and Analyze DNA Sequencing Files from the Cancer Center

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A39 Isolation of Bacterial Genomic DNA

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A40 Protocol for Using Wizard Plus SV Minipreps Kit

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A41 How to Prepare homemade MB grade Glycogen

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A42 DNA Gel Extraction using MIDSCI Kit (by Wei Jiang)

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A43 Protocol for Site-Directed Mutagenesis

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A44 Mouse Tail DNA isolation (updated by Sean)

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A45 High Speed Plasmid Miniprep using MIDSCI kit (by Wei Jiang)

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A46 New NEB Buffer Chart for commonly used restriction enzymes (by Liang Chen)

 

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