THE UNIVERSITY OF CHICAGO MEDICAL CENTER

MOLECULAR ONCOLOGY LABORATORY

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Laboratory Protocols

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C.  RNA Techniques

 

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C1 Protocol for total RNA isolation using RNAgent kit

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C2 Preparing and running Northern blotting gel

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C3 Northern Blotting Gel Transfer and Hybridization

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C4 Revised NB protocol-use of Hybridization Oven

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C5 Protocol for Preparation of Oligo(dT)-Beads

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C6 Total RNA Isolation Using RNAgent Kit (by Kang)

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C7 Total RNA preparation Using TRIzol reagent

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C8 IN SITU HYBRIDIZATION - FROZEN SECTIONS (Polyak Lab)

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C9 How to Produce and Use the siRNA Cocktails for RNAi Knockdown

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C10 How to isolate RNA from tissue samples (by Xiaoji Luo)

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C11 Preparation of RT-PCR for qPCR (by Xiaoji Luo)

 

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