MOLECULAR ONCOLOGY LABORATORY
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Laboratory Protocols
E. Cell and Tissue Culture Techniques
E1 Procedure for Passage of Mammalian Cells
E2 Procedure for transfection of mammalian cells using LipofectAMINE
E3 Protocol for Freezing and Thawing Mammalian cells
E4 Procedure for establishing stable clones of mammalian cells
E5 Luciferase reporters made with pBV-Luc
E6 Beta-Galactosidase (X-Gal) Staining Assay
E7 Protocol for total crystal violet staining of mammalian cells
E8 Protocol for preparing complete cell culture medium and G418-containing medium
E9 AdEasy Got Easier
E10 Hoechst 33258 Staining
E11 Prepare Genomic DNA from Mammalian Cell lines
E12 Hirt protocol for isolating small DNA molecules from mammalian cells
E13 Preparation of BMP Conditioned Medium
E14 Transfection of 293 cells for making adenovirus
E15 MTS (Promega) Cell Proliferation Assay
E16 Transwell (Boyden Chamber) Cell Migration Assay
E17 AdEasy Comprehensive Protocol from CPHG
E18 MTT Cell Proliferation Assay
E19 Luciferase Reporter Assay
E20 Mouse embryonic fibroblast (MEFs) isolation
E21 Quantitative fluorescence measurement using Modulus
E22 Drug selection table for commonly used lines (by Chris Song)
E23 How to Select Optimal siRNA Target Sites Using SOS-Based Vectors
E24 Retrovirus production and establishment of stable cell lines
E25 Collagen Gel Colony Formation Assay
E26 FACS Cell cycle analysis (by Xiaoji Luo)
E27 Promega beta-Gal assay
E28 Collagen coating of tissue culture dishes
E29 Transwell Cell Migration-Invasion Matrigel Assays (by Yuxi Su)
E30 How to eliminate Mycoplasma from infected cells using Plasmocin (Invivogen)
E31 How to Coat Cell Culture Plasticware with 0.1% Gelatin (Wenli Zhang)
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