Hoechst 33258 Staining

 

 

Lan Zhou  2/7/03

 

 

Collect the medium to 15 ml tube;

Trypsinize the cells and transfer it into that 15ml tube;

Spin down for 5mins;

Remove the sup.

Add 10ul PBS into the tube;

Mix by pipetting up and down;

Transfer it to a new 1.5ml tube that contains the magic solution;

Mix and store it @ 4C or smear it on glass plate directly

Check it under microscope

 

 

Magic solution:

 

75ul             PBS;

5ul               10% NP-40 in PBS;

9ul               37% formalhehyde;

1ul               1mg/ml Hoechst 33258 (Molecular Probes)