Hoechst 33258 Staining

Lan Zhou 2/7/03

Collect the medium to 15 ml tube;

Trypsinize the cells and transfer it into that 15ml tube;

Spin down for 5mins;

Remove the sup.

Add 10ul PBS into the tube;

Mix by pipetting up and down;

Transfer it to a new 1.5ml tube that contains the magic solution;

Mix and store it @ 4C or smear it on glass plate directly

Check it under microscope

Magic solution:

75ul PBS;

5ul 10% NP-40 in PBS;

9ul 37% formalhehyde;

1ul 1mg/ml Hoechst 33258 (Molecular Probes)