The University of Chicago Medical Center

	THE UNIVERSITY OF CHICAGO MEDICAL CENTER MOLECULAR ONCOLOGY LABORATORY

Molecular Oncology Laboratory Protocols

	Molecular Oncology Lab Biosafety Manual
	Molecular Oncology Lab Guidelines for Common Practices
	Molecular Oncology Lab Unconventional Cloning Tips
	A. Bacterial, DNA & Cloning Techniques
	B. PCR Techniques
	C. RNA Techniques
	D. Protein Techniques
	E. Tissue & Cell Culture Techniques
	F. Skeletal & Stem Cell Biology & Histology Techniques
	G. Instrument Use & Procedures
	H. Solutions, Buffers & Miscellaneous
	Molecular Oncology Lab Graduation Checklist

Cloning and Expression Vectors

pAdEasy Vectors	pCMV-NK-HAC	pRV-Linker	pSEB-Linker
pAdTrack-TO4 (eGFP)	pCMV-CFT (Flag)	pRV-RFP	pSEB-Flag
pAdTrace-TO4 (mRFP)	pCMV-CHA (HA)	pRV-TO4	pSEN-Flag
pShuttle-RFP (mRFP)	pCMV-CHis (6xHis)	pSCV-Linker	pSEH-Flag
pSES-HUS (AdV siRNA, pSES)	pCMV-CMT (Myc)	pSCV-BsdRFP	pSEZ-Flag
pShuttle-LG (LoxP-GFP)	pCMV-hFc (IgG)	pSCV-TOFT (Flag)
	pCMV-GLuc (Gaussia Luciferase)	pSCV-TOHis (6xHis)	pSEB-3F (3x Flag)
pSOS-HUS (non-RV,Sfi I)	pCMV-CRFP (red FP)	pSCV-TOFc (IgG)	pSEB-3H (3x 6His)
pSOS-HUD (non-RV, Xcm I)	pCMV-CFP (cyan FP)	pRV-TO5	pSEB-3M (3x Myc)
pTOP-Luc	pCMV-CAP (SEAP)	pRV-TOMT (Myc)
pBV-Luc (Luc reporter)	pCMV-CFT2 (Flag)	pRV-TOFT (Flag)	pSEB-CG (C-terminal copGFP)
	pCMV-CHA2 (HA)	pRV-TOHis (6xHis)	pSEB-NG (N-terminal copGFP)
pMOLUC	pCMV-CHis2 (6xHis)
pMOK	pCMV-CMT2 (Myc)	pBG2Luc (GLuc-cGFP reporter)	pSEB-CBT (C-terminal biotin tag)
pTLOX	pCMV-hFc2 (IgG)	pBGLuc (GLuc reporter)	pSEB-NBT (N-terminal biotin tag)
pTHE	pCMV-CePF (Cerulean)
pTRE	pCMV-OFP (mKO1)	pBRS (RheoSwitch inducible)	pSEB-HUS (Sfi I) (RV siRNA = pSOS)
pGST-MOLUC	pCMV-YFP (Venus)	pHRS (RheoSwitch inducible)	pSEH-HUS (Sfi I) (RV siRNA)
pMRFP-C1	pSCV-Flag	pNRS (RheoSwitch inducible)	pSEN-HUS (Sfi I) (RV siRNA)
pHAHA	pSCV-His	pPRS (RheoSwitch inducible)
	pSCX-Flag		pSEB-HUD (Xcm I) (RV siRNA)
	pSCX-His

Commercial Vectors

Requested Vectors