The University of Chicago Medical Center

	THE UNIVERSITY OF CHICAGO MEDICAL CENTER MOLECULAR ONCOLOGY LABORATORY

Molecular Oncology Laboratory Protocols

	MOLab Biosafety Manual
	MOLab Survival Manual
	MOLab "Law and Order"
	MOLab Unconventional Cloning Tips
	A. Bacterial, DNA & Cloning Techniques
	B. PCR Techniques
	C. RNA Techniques
	D. Protein Techniques
	E. Tissue & Cell Culture Techniques Hood Sign-up sheet Cell Culture Basics Videos V-1 V-2 V-3 V-4 V-5
	F. Skeletal & Stem Cell Biology & Histology Techniques
	G. Instrument Use & Procedures
	H. Solutions, Buffers & Miscellaneous
	Molecular Oncology Lab Graduation Checklist

Cloning and Expression Vectors

UCMOLab Vectors MOLab Vector Template MOLab Construct Submission Form

pAdEasy Vectors	pCMV-NK-HAC	pRV-Linker	pSEB-Linker
pAdTrack-TO4 (eGFP)	pCMV-CFT (Flag)	pRV-RFP	pSEB-Flag
pAdTrack-TOX (eGFP)	pCMV-CHA (HA)	pRV-TO4	pSEN-Flag
pAdTrace-TOX (mRFP)	pCMV-CHis (6xHis)	pSCV-Linker	pSEH-Flag
pAdTrace-TO4 (mRFP)	pCMV-CMT (Myc)	pSCV-BsdRFP	pSEZ-Flag
pShuttle-RFP (mRFP)	pCMV-hFc (IgG)	pSCV-TOFT (Flag)
pSES (AdV siRNA, pSES-HUS)	pCMV-GLuc (Gaussia Luciferase)	pSCV-TOHis (6xHis)	pSEB-C3F (C-terminal 3x Flag)
pSES1 (AdV siRNA, 09/09/09)	pCMV-CRFP (red FP)	pSCV-TOFc (IgG)	pSEB-N3F (N-terminal 3x Flag)
pShuttle-LG (LoxP-GFP)	pCMV-CFP (cyan FP)	pRV-TO5	pSEB-3H (3x 6His)
pASG (AdV shuttle w/ copGFP)	pCMV-CAP (SEAP)	pRV-TOMT (Myc)	pSEB-3M (3x Myc)
pASG2 (AdV w/ 2x CMV-PA)	pCMV-CFT2 (Flag)	pRV-TOFT (Flag)
pAIR (Adv RheoSwitch inducible)	pCMV-CHA2 (HA)	pRV-TOHis (6xHis)	pSBC-3F (CMV)
pAdHD-Linker (helper-dependent)	pCMV-CHis2 (6xHis)
pSOS-HUS (non-RV,Sfi I)	pCMV-CMT2 (Myc)	pBG2Luc (GLuc-cGFP reporter)	pSEB-CBT (C-terminal biotin tag)
pSOS-HUD (non-RV, Xcm I)	pCMV-hFc2 (IgG)	pBGLuc (GLuc reporter)	pSEB-NBT (N-terminal biotin tag)
pTOP-Luc	pCMV-CePF (Cerulean)	pBRS (RheoSwitch inducible)
pBV-Luc (Luc reporter)	pCMV-OFP (mKO1)	pHRS (RheoSwitch inducible)	pSEB-HUS (Sfi I) (RV siRNA = pSOS)
pMOLUC (cloning vector)	pCMV-YFP (Venus)	pNRS (RheoSwitch inducible)	pSEH-HUS (Sfi I) (RV siRNA)
pMOK (cloning vector)	pSCV-Flag pSCX-Flag	pPRS (RheoSwitch inducible)	pSEN-HUS (Sfi I) (RV siRNA)
pTLOX	pSCV-His pSCX-His
pTHE		pSEB-GLuc1 (PCA assay)	pSEB-HUD (Xcm I) (RV siRNA)
pTRE		pSEB-GLuc2 (PCA assay)
pGST-MOLUC	pMPB2 (piggybac)		pSEB-CG (C-terminal copGFP)
pmRFP-C1		pSEB-Display	pSEB-NG (N-terminal copGFP)
pHAHA

Commercial Vectors

pDisplay	pEGFP-C1	pEGFP-N1	pcDNA3.1
pCMVb	pGEX vectors	pBlueScript II KS	pUC19
pCEP4	pCMV-SPORT 6	pDNR-LIB	pOTB7
pCI-neo	pGL3-Control	pBI-GL	pBI tet vector
pCMVb	pBR322	pC
Huge Vector List

Requested Vectors

pCS2+	pCCALL2 Seq	pCCALL2-Z/EG Seq

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